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B cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunization
Fontoura, I. C.; Trombone, A.P.F.; Almeida, L. P.; Lorenzi, J. C. C.; Rossetti, R. A. M.; Malardo, T.; Padilha, E.; Schluchting, W.; Silva, R. L. L.; Gembre, A. F.; Fiuza, J. E. C.; Silva, C. L.; Panunto-Castelo, A.; Coelho-Castelo, A. A. M..
  • Fontoura, I. C.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Trombone, A.P.F.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Almeida, L. P.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Lorenzi, J. C. C.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Rossetti, R. A. M.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Malardo, T.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Padilha, E.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Schluchting, W.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Silva, R. L. L.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Gembre, A. F.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Fiuza, J. E. C.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Silva, C. L.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Panunto-Castelo, A.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
  • Coelho-Castelo, A. A. M.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto. BR
Braz. j. med. biol. res ; 48(12): 1095-1100, Dec. 2015. graf
Article in English | LILACS | ID: lil-762920
Responsible library: BR1.1
ABSTRACT
In DNA vaccines, the gene of interest is cloned into a bacterial plasmid that is engineered to induce protein production for long periods in eukaryotic cells. Previous research has shown that the intramuscular immunization of BALB/c mice with a naked plasmid DNA fragment encoding the Mycobacterium leprae 65-kDa heat-shock protein (pcDNA3-Hsp65) induces protection against M. tuberculosis challenge. A key stage in the protective immune response after immunization is the generation of memory T cells. Previously, we have shown that B cells capture plasmid DNA-Hsp65 and thereby modulate the formation of CD8+ memory T cells after M. tuberculosis challenge in mice. Therefore, clarifying how B cells act as part of the protective immune response after DNA immunization is important for the development of more-effective vaccines. The aim of this study was to investigate the mechanisms by which B cells modulate memory T cells after DNA-Hsp65 immunization. C57BL/6 and BKO mice were injected three times, at 15-day intervals, with 100 µg naked pcDNA-Hsp65 per mouse. Thirty days after immunization, the percentages of effector memory T (TEM) cells (CD4+ and CD8+/CD44high/CD62Llow) and memory CD8+ T cells (CD8+/CD44high/CD62Llow/CD127+) were measured with flow cytometry. Interferon γ, interleukin 12 (IL-12), and IL-10 mRNAs were also quantified in whole spleen cells and purified B cells (CD43−) with real-time qPCR. Our data suggest that a B-cell subpopulation expressing IL-10 downregulated proinflammatory cytokine expression in the spleen, increasing the survival of CD4+ TEM cells and CD8+ TEM/CD127+ cells.
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Full text: Available Index: LILACS (Americas) Main subject: RNA, Messenger / B-Lymphocytes / T-Lymphocyte Subsets / Immunomodulation / Heat-Shock Proteins Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: RNA, Messenger / B-Lymphocytes / T-Lymphocyte Subsets / Immunomodulation / Heat-Shock Proteins Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR