Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area
Mem. Inst. Oswaldo Cruz
;
110(6): 814-816, Sept. 2015. tab, graf
Article
in English
| LILACS
| ID: lil-763088
ABSTRACT
Currently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we report the first genome sequencing of a P. vivaxisolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivaxSalvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novoassembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known virgenes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research onP. vivaxpopulation genetics.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plasmodium vivax
/
DNA, Protozoan
/
Sequence Analysis, DNA
/
Genome, Protozoan
Country/Region as subject:
Asia
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
National Institute of Parasitic Diseases/CN
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