Characterization of reactive stroma in prostate cancer: involvement of growth factors, metalloproteinase matrix, sexual hormones receptors and prostatic stem cells
Int. braz. j. urol
;
41(5): 849-858, Sept.-Oct. 2015. tab, graf
Article
in English
| LILACS
| ID: lil-767051
ABSTRACT
ABSTRACT Introduction and Objectives:
Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. Materials andMethods:
Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups Group-1 (n=20) samples without reactive stroma; Group-2 (n=20) samples of PCa with intense stroma reaction. Prostatic samples were evaluated for RStr intensity by Masson Trichromic stain and posteriorly submitted to histopathological and immunohistochemistry analysis for antigens α-actin, vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA, AR, Erα and ERβ.Results:
Reactive stroma with intense desmoplastic reactivity was significantly more frequent in intermediate (Gleason 7, 3+4) and high grade tumors (Gleason 7, 4+3). The group with intense stromal reactivity showed significant higher levels of Vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA and ERα.Conclusions:
It can be concluded that RStr may be a predictive marker of Pca progression, since it was associated with increase of growth factors, imbalance of androgen and estrogen receptors and presence of malign prostatic stem cells.
Full text:
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Index:
LILACS (Americas)
Main subject:
Prostatic Neoplasms
/
Neoplastic Stem Cells
/
Adenocarcinoma
/
Stromal Cells
/
Epithelial Cells
Type of study:
Prognostic study
Limits:
Aged
/
Aged80
/
Humans
/
Male
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2015
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas/BR
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