Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil
Braz. j. microbiol
;
46(4): 1217-1223, Oct.-Dec. 2015. tab, graf
Article
in English
| LILACS
| ID: lil-769643
ABSTRACT
Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point was developed to analyze the effects and interactions between initial pH, temperature and the concentration of gelatin. Based on the results of the first 23 full factorial design, a successive 23 full factorial design was performed. The most favorable production conditions were found to be 1.5% (w/v) gelatin, pH 9.0 and 37 °C with enzymatic activity of 86.27 U/mL. The enzyme showed optimal activity at 50 °C and pH 9.0, and it was stable over wide pH (7.2-10.0) and temperature (45 °C-60 °C) ranges. These results indicate that Bacillus sp DPUA 1728 is a potential source for producing collagenolytic protease with possible biotechnological applications, such as in the food, cosmetics and leather industries.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Social Support
/
Exercise
/
Residence Characteristics
/
City Planning
/
Environment Design
Type of study:
Controlled clinical trial
/
Diagnostic study
/
Qualitative research
Limits:
Adolescent
/
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade do Estado do Amazonas/BR
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