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Production of Brazilian human norovirus VLPs and comparison of purification methods
Lamounier, Thais Alves da Costa; Oliveira, Layssa Miranda de; Camargo, Brenda Rabello de; Rodrigues, Kelly Barreto; Noronha, Eliane Ferreira; Ribeiro, Bergmann Morais; Nagata, Tatsuya.
  • Lamounier, Thais Alves da Costa; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
  • Oliveira, Layssa Miranda de; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
  • Camargo, Brenda Rabello de; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
  • Rodrigues, Kelly Barreto; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
  • Noronha, Eliane Ferreira; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
  • Ribeiro, Bergmann Morais; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
  • Nagata, Tatsuya; Universidade de Brasília. Departamento de Farmácia. Brasília. BR
Braz. j. microbiol ; 46(4): 1265-1268, Oct.-Dec. 2015. graf
Article in English | LILACS | ID: lil-769661
ABSTRACT
Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
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Full text: Available Index: LILACS (Americas) Main subject: Viral Structural Proteins / Centrifugation, Density Gradient / Chromatography, Ion Exchange / Virosomes / Norovirus Limits: Child / Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de Brasília/BR

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Full text: Available Index: LILACS (Americas) Main subject: Viral Structural Proteins / Centrifugation, Density Gradient / Chromatography, Ion Exchange / Virosomes / Norovirus Limits: Child / Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de Brasília/BR