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Mutational and acquired carbapenem resistance mechanisms in multidrug resistant Pseudomonas aeruginosa clinical isolates from Recife, Brazil
Cavalcanti, Felipe Lira de Sá; Mirones, Cristina Rodríguez; Paucar, Elena Román; Montes, Laura Álvarez; Leal-Balbino, Tereza Cristina; Morais, Marcia Maria Camargo de; Martínez-Martínez, Luis; Ocampo-Sosa, Alain Antonio.
  • Cavalcanti, Felipe Lira de Sá; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Mirones, Cristina Rodríguez; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Paucar, Elena Román; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Montes, Laura Álvarez; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Leal-Balbino, Tereza Cristina; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Morais, Marcia Maria Camargo de; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Martínez-Martínez, Luis; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
  • Ocampo-Sosa, Alain Antonio; Universidade de Pernambuco. Instituto de Ciências Biológicas. Laboratório de Resistência Microbiana. Recife. BR
Mem. Inst. Oswaldo Cruz ; 110(8): 1003-1009, Dec. 2015. tab, graf
Article in English | LILACS | ID: lil-769825
ABSTRACT
An investigation was carried out into the genetic mechanisms responsible for multidrug resistance in nine carbapenem-resistant Pseudomonas aeruginosaisolates from different hospitals in Recife, Brazil. Susceptibility to antimicrobial agents was determined by broth microdilution. Polymerase chain reaction (PCR) was employed to detect the presence of genes encoding β-lactamases, aminoglycoside-modifying enzymes (AMEs), 16S rRNA methylases, integron-related genes and OprD. Expression of genes coding for efflux pumps and AmpC cephalosporinase were assessed by quantitative PCR. The outer membrane proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The blaSPM-1, blaKPC-2 and blaGES-1 genes were detected in P. aeruginosaisolates in addition to different AME genes. The loss of OprD in nine isolates was mainly due to frameshift mutations, premature stop codons and point mutations. An association of loss of OprD with the overexpression of MexAB-OprM and MexXY-OprM was observed in most isolates. Hyper-production of AmpC was also observed in three isolates. Clonal relationship of the isolates was determined by repetitive element palindromic-PCR and multilocus sequence typing. Our results show that the loss of OprD along with overexpression of efflux pumps and β-lactamase production were responsible for the multidrug resistance in the isolates analysed.
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Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas aeruginosa / Beta-Lactamases / Carbapenems / Beta-Lactam Resistance / Drug Resistance, Multiple, Bacterial / Mutation Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de Pernambuco/BR

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Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas aeruginosa / Beta-Lactamases / Carbapenems / Beta-Lactam Resistance / Drug Resistance, Multiple, Bacterial / Mutation Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2015 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de Pernambuco/BR