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Polyphasic characterisation of Burkholderia cepaciacomplex species isolated from children with cystic fibrosis
Vicenzi, Fernando José; Pillonetto, Marcelo; Souza, Helena Aguilar Peres Homem de Mello de; Palmeiro, Jussara Kasuko; Riedi, Carlos Antônio; Rosario-Filho, Nelson Augusto; Dalla-Costa, Libera Maria.
  • Vicenzi, Fernando José; Laboratório Municipal de Curitiba. Curitiba. BR
  • Pillonetto, Marcelo; Laboratório Municipal de Curitiba. Curitiba. BR
  • Souza, Helena Aguilar Peres Homem de Mello de; Laboratório Municipal de Curitiba. Curitiba. BR
  • Palmeiro, Jussara Kasuko; Laboratório Municipal de Curitiba. Curitiba. BR
  • Riedi, Carlos Antônio; Laboratório Municipal de Curitiba. Curitiba. BR
  • Rosario-Filho, Nelson Augusto; Laboratório Municipal de Curitiba. Curitiba. BR
  • Dalla-Costa, Libera Maria; Laboratório Municipal de Curitiba. Curitiba. BR
Mem. Inst. Oswaldo Cruz ; 111(1): 37-42, Jan. 2016. tab
Article in English | LILACS | ID: lil-771076
ABSTRACT
Cystic fibrosis (CF) patients with Burkholderia cepacia complex (Bcc) pulmonary infections have high morbidity and mortality. The aim of this study was to compare different methods for identification of Bcc species isolated from paediatric CF patients. Oropharyngeal swabs from children with CF were used to obtain isolates of Bcc samples to evaluate six different tests for strain identification. Conventional (CPT) and automatised (APT) phenotypic tests, polymerase chain reaction (PCR)-recA, restriction fragment length polymorphism-recA, recAsequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) were applied. Bacterial isolates were also tested for antimicrobial susceptibility. PCR-recA analysis showed that 36 out of the 54 isolates were Bcc. Kappa index data indicated almost perfect agreement between CPT and APT, CPT and PCR-recA, and APT and PCR-recA to identify Bcc, and MALDI-TOF and recAsequencing to identify Bcc species. The recAsequencing data and the MALDI-TOF data agreed in 97.2% of the isolates. Based on recA sequencing, the most common species identified were Burkholderia cenocepacia IIIA (33.4%),Burkholderia vietnamiensis (30.6%), B. cenocepaciaIIIB (27.8%), Burkholderia multivorans (5.5%), and B. cepacia (2.7%). MALDI-TOF proved to be a useful tool for identification of Bcc species obtained from CF patients, although it was not able to identify B. cenocepacia subtypes.
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Full text: Available Index: LILACS (Americas) Main subject: Burkholderia Infections / Cystic Fibrosis / Burkholderia cepacia complex Limits: Child / Child, preschool / Female / Humans / Infant / Male Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Laboratório Municipal de Curitiba/BR

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Full text: Available Index: LILACS (Americas) Main subject: Burkholderia Infections / Cystic Fibrosis / Burkholderia cepacia complex Limits: Child / Child, preschool / Female / Humans / Infant / Male Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: Laboratório Municipal de Curitiba/BR