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A molecular platform for the diagnosis of multidrug-resistant and pre-extensively drug-resistant tuberculosis based on single nucleotide polymorphism mutations present in Colombian isolates of Mycobacterium tuberculosis
Martínez, Luz Maira Wintaco; Castro, Gloria Puerto; Guerrero, Martha Inírida.
  • Martínez, Luz Maira Wintaco; Instituto Nacional de Salud. Bogotá. CO
  • Castro, Gloria Puerto; Instituto Nacional de Salud. Bogotá. CO
  • Guerrero, Martha Inírida; Instituto Nacional de Salud. Bogotá. CO
Mem. Inst. Oswaldo Cruz ; 111(2): 93-100, Feb. 2016. tab, graf
Article in English | LILACS | ID: lil-772615
ABSTRACT
Developing a fast, inexpensive, and specific test that reflects the mutations present in Mycobacterium tuberculosis isolates according to geographic region is the main challenge for drug-resistant tuberculosis (TB) control. The objective of this study was to develop a molecular platform to make a rapid diagnosis of multidrug-resistant (MDR) and extensively drug-resistant TB based on single nucleotide polymorphism (SNP) mutations present in therpoB, katG, inhA,ahpC, and gyrA genes from Colombian M. tuberculosis isolates. The amplification and sequencing of each target gene was performed. Capture oligonucleotides, which were tested before being used with isolates to assess the performance, were designed for wild type and mutated codons, and the platform was standardised based on the reverse hybridisation principle. This method was tested on DNA samples extracted from clinical isolates from 160 Colombian patients who were previously phenotypically and genotypically characterised as having susceptible or MDR M. tuberculosis. For our method, the kappa index of the sequencing results was 0,966, 0,825, 0,766, 0,740, and 0,625 forrpoB, katG, inhA,ahpC, and gyrA, respectively. Sensitivity and specificity were ranked between 90-100% compared with those of phenotypic drug susceptibility testing. Our assay helps to pave the way for implementation locally and for specifically adapted methods that can simultaneously detect drug resistance mutations to first and second-line drugs within a few hours.
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Full text: Available Index: LILACS (Americas) Main subject: DNA, Bacterial / Tuberculosis, Multidrug-Resistant / Polymorphism, Single Nucleotide / Molecular Diagnostic Techniques / Mutation / Mycobacterium tuberculosis Type of study: Diagnostic study Limits: Humans Country/Region as subject: South America / Colombia Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2016 Type: Article / Project document Affiliation country: Colombia Institution/Affiliation country: Instituto Nacional de Salud/CO

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Full text: Available Index: LILACS (Americas) Main subject: DNA, Bacterial / Tuberculosis, Multidrug-Resistant / Polymorphism, Single Nucleotide / Molecular Diagnostic Techniques / Mutation / Mycobacterium tuberculosis Type of study: Diagnostic study Limits: Humans Country/Region as subject: South America / Colombia Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2016 Type: Article / Project document Affiliation country: Colombia Institution/Affiliation country: Instituto Nacional de Salud/CO