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The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
Lv, Yuntong; Xia, Zhikuan; Wang, Wenling.
  • Lv, Yuntong; General Hospital of Beijing Military Command of PLA. Department of Dermatology. Beijing. CN
  • Xia, Zhikuan; General Hospital of Beijing Military Command of PLA. Department of Dermatology. Beijing. CN
  • Wang, Wenling; General Hospital of Beijing Military Command of PLA. Department of Dermatology. Beijing. CN
Braz. j. microbiol ; 47(1): 266-269, Jan.-Mar. 2016. graf
Article in English | LILACS | ID: lil-775127
ABSTRACT
Abstract The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers were present at the tip of the budding site of the spore, blastospore, and the germ tube of T. asahii. We also found that Spitzenkörpers were present at the tip of the hyphae as well as the subapical regions. Cytochalasin D, an inhibitor of actin polymerization, leads to abnormal Spitzenkörper formation and loss of cell polarity.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Pyridinium Compounds / Staining and Labeling / Trichosporon / Organelles / Hyphae / Fluorescent Dyes / Quaternary Ammonium Compounds Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2016 Type: Article / Project document Affiliation country: China Institution/Affiliation country: General Hospital of Beijing Military Command of PLA/CN

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Full text: Available Index: LILACS (Americas) Main subject: Pyridinium Compounds / Staining and Labeling / Trichosporon / Organelles / Hyphae / Fluorescent Dyes / Quaternary Ammonium Compounds Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2016 Type: Article / Project document Affiliation country: China Institution/Affiliation country: General Hospital of Beijing Military Command of PLA/CN