Development of an ex vivo model of endovenous laser ablation of the great saphenous vein in a pilot study
Acta cir. bras
;
31(3): 161-167, Mar. 2016. graf
Article
in English
| LILACS
| ID: lil-777093
ABSTRACT
ABSTRACT PURPOSE:
To develop an ex vivo model for the analysis of macroscopic, histological and immunohistochemical changes after experimental endovenous laser ablation (EVLA) of the great saphenous vein (GSV).METHODS:
We describe a model produced with glass tubes and introducer sheaths to mimic the physiological conditions of EVLA procedures, such as tumescence and blood flow. A pilot study was conducted to evaluate an ex vivo procedure of EVLA of an incompetent GSV segment using a 1470-nm radial fiber diode laser (7 W power) and an automatic pull-back device. The vein segment was analyzed macroscopically and by hematoxylin & eosin staining, elastic fiber histochemistry, Gomori's trichrome staining, and alpha-smooth muscle actin immunohistochemistry.RESULTS:
No perforations were observed macroscopically. No muscle cell adhesion was observed in the central part of the ablated vein, showing tissue disruption. There was low labeling for elastic fibers, disruption of muscle fibers, and a reduced expression of the specific marker for this cell type.CONCLUSION:
This ex vivo endovenous laser ablation model is a low cost alternative to in vivo experiments, providing standardized experimental conditions.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Saphenous Vein
/
Laser Therapy
/
Lasers, Semiconductor
/
Muscle, Smooth, Vascular
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2016
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Paraná/BR
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