Allopurinol preconditioning attenuates renal ischemia/reperfusion injury by inhibiting HMGB1 expression in a rat model
Acta cir. bras
;
31(3): 176-182, Mar. 2016. graf
Article
in English
| LILACS
| ID: lil-777094
ABSTRACT
ABSTRACT PURPOSE:
To investigate the potential effects of pretreatment with allopurinol on renal ischemia/reperfusion injury (IRI) in a rat model.METHODS:
Twenty four rats were subjected to right kidney uninephrectomy were randomly distributed into the following three groups (n=8) Group A (sham-operated group); Group B (ischemic group) with 30 min of renal ischemia after surgery; and Group C (allopurinol + ischemia group) pretreated with allopurinol at 50 mg/kg for 14 days. At 72 h after renal reperfusion, the kidney was harvested to assess inflammation and apoptosis.RESULTS:
Pretreatment with allopurinol significantly improved renal functional and histological grade scores following I/R injury (p<0.05). Compared with Group B, the expression levels of caspase-3 and Bax were markedly reduced in Group C, meanwhile, whereas expression of bcl-2 was clearly increased (p<0.05). A newly described marker of inflammation, High Mobility Group Box 1(HMGB1), showed reduced expression in Group C (p<0.05).CONCLUSION:
Pretreatment with allopurinol had a protective effect on kidney ischemia/reperfusion injury, which might be related to the inhibition of HMGB1 expression.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Reperfusion Injury
/
Allopurinol
/
Ischemic Preconditioning
/
Protective Agents
/
HMGB1 Protein
/
Kidney
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2016
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Wuhan University/CN
Similar
MEDLINE
...
LILACS
LIS