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Influence of Temperature on the Antibacterial Activity of Sodium Hypochlorite
Giardino, Luciano; Mohammadi, Zahed; Beltrami, Riccardo; Poggio, Claudio; Estrela, Carlos; Generali, Luigi.
  • Giardino, Luciano; Private Practice. Crotone. IT
  • Mohammadi, Zahed; Private Practice. Crotone. IT
  • Beltrami, Riccardo; Private Practice. Crotone. IT
  • Poggio, Claudio; Private Practice. Crotone. IT
  • Estrela, Carlos; Private Practice. Crotone. IT
  • Generali, Luigi; Private Practice. Crotone. IT
Braz. dent. j ; 27(1): 32-36, Jan.-Feb. 2016. tab, graf
Article in English | LILACS | ID: lil-777145
ABSTRACT
Abstract The aim of this study was to compare the antimicrobial activity of 5.25% NaOCl, Hypoclean and Chlor-Xtra at 20 °C and 45 °C in bovine root dentin. One-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with Enterococcus faecalis. The specimens were divided into the following groups 1. 5.25% NaOCl 20 °C; 2. Hypoclean 20 °C; 3. Chlor-Xtra 20 °C; 4. 5.25% % NaOCl 45 °C; 5. Hypoclean 45 °C; 6. Chlor-Xtra 45 °C; 7. positive control; 8. negative control. Dentin chips were collected with round burs into Brain Heart Infusion (BHI) broth. After culturing, the number of colony-forming units (CFU) was counted. Statistical analyses were performed using descriptive statistics (mean, standard deviation, median), Shapiro-Wilk test, ANOVA and Tukey test. Significance level was set at p<0.05. In all experimental groups, CFU was minimum after treatment (day 0) and the obtained results were significantly different from each other at any period (p<0.05). After treatment, the Hypoclean and Chlor-Xtra showed the lowest numbers of CFU at 20 °C and 45 °C, whereas 5.25% NaOCl showed the highest number of CFU at both temperatures. In each group, the number of CFUs increased significantly with time (p<0.05). The antibacterial activity of Hypoclean and Chlor-Xtra at 45 °C were significantly greater than other tested solutions.
RESUMO
Resumo O objetivo deste estudo foi comparar a atividade antimicrobiana do hipoclorito de sódio a 5,25%, Hypoclean e Cloro-Xtra a 20 °C e 45 °C em dentina radicular bovina. Um total de 170 tubos de dentina foram preparados a partir de incisivos superiores bovinos infectados por 21 dias com Enterococcus faecalis. Os espécimes foram divididos nos seguintes grupos 1. NaOCl - 5,25% a 20 °C; 2. Hypoclean 20 °C; 3. Cloro-Xtra 20 °C; 4. NaOCl - 5,25% a 45 °C; 5. Hypoclean 45 °C; 6. Cloro-Xtra 45 °C; 7. Controle positivo; 8. Controle negativo. Raspas de dentina foram coletadas com brocas esféricas e cultivadas em infusão cérebro coração (brain heart infusion - BHI). Após a cultura, o número de unidades formadoras de colônias (UFC) foi contado. As análises estatísticas foram realizadas utilizando estatística descritiva (média, desvio padrão, mediana), teste de Shapiro-Wilk, ANOVA e teste de Tukey. O nível de significância foi estabelecido p<0,05. Em todos os grupos experimentais, o número de UFC foi mínimo após o tratamento inicial. Os resultados obtidos foram significativamente diferentes nos períodos de tempos experimentais (p<0,05). O Hypoclean e o Cloro-Xtra mostraram o menor número de UFC a 20 °C e a 45 °C, enquanto que o NaOCl - 5,25% apresentou maior número de UFC em ambas as temperaturas. Em cada grupo, o número de CFUs foi significativamente aumentado por período de tempo (p<0,05). As atividades antibacterianas do Hypoclean e Chlor-Xtra a 45 °C foram significativamente maiores do que nas outras soluções testadas.
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Full text: Available Index: LILACS (Americas) Main subject: Sodium Hypochlorite / Temperature / Anti-Bacterial Agents Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Italy Institution/Affiliation country: Private Practice/IT

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Full text: Available Index: LILACS (Americas) Main subject: Sodium Hypochlorite / Temperature / Anti-Bacterial Agents Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Italy Institution/Affiliation country: Private Practice/IT