Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage
Braz. oral res. (Online)
;
29(1): 1-7, 2015. tab, ilus
Article
in English
| LILACS
| ID: lil-777235
ABSTRACT
The objective of this study was to describe a new method for the quantitative analysis of a microleakage of endodontic filling materials. Forty extracted single-rooted teeth were randomly divided into three experimental groups. After root canal shaping, the experimental groups were filled using the lateral condensation technique with the Epiphany system (G1), with gutta-percha + Sealapex (G2), and with gutta-percha + AH Plus (G3). Each root was mounted on a modified leakage testing device, and caffeine solution was used as a tracer (2000 ng mL-1, pH 6.0), applied in the coronal direction towards the tooth apex, creating a hydrostatic pressure of 2.55 kPa. Presence of caffeine in the receiving solution was measured after 10, 30, and 60 days, using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). None of the groups presented microleakage at 10 days. At 30 days, G2 and G3 showed similar infiltration patterns (means 16.0 and 13.9 ng mL-1, respectively), whereas G1 showed significantly higher values (mean 105.2 ng mL-1). At 60 days, leakage values were 182.6 ng mL-1for G1, 139.0 ng mL-1 for G2, and 53.5 ng mL-1 for G3. AH Plus showed the best sealing ability and HPLC-MS/MS showed high sensitivity and specificity for tracer quantification.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Root Canal Obturation
/
Root Canal Preparation
/
Dental Leakage
Type of study:
Controlled clinical trial
Limits:
Humans
Language:
English
Journal:
Braz. oral res. (Online)
Journal subject:
Dentistry
Year:
2015
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Tuiuti do Paraná/BR
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