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Effects of epicatechin, a crosslinking agent, on human dental pulp cells cultured in collagen scaffolds
Lim, Eun-su; Lim, Myung-Jin; Min, Kyung-San; Kwon, Young-Sun; Hwang, Yun-Chan; Yu, Mi-Kyung; Hong, Chan-Ui; Lee, Kwang-Won.
  • Lim, Eun-su; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Lim, Myung-Jin; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Min, Kyung-San; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Kwon, Young-Sun; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Hwang, Yun-Chan; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Yu, Mi-Kyung; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Hong, Chan-Ui; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
  • Lee, Kwang-Won; Chonbuk National University. School of Dentistry. Institute of Oral Bioscience. Jeonju. KR
J. appl. oral sci ; 24(1): 76-84, Jan.-Feb. 2016. graf
Article in English | LILACS, BBO | ID: lil-777354
ABSTRACT
ABSTRACT Objective The purpose of this study was to investigate the biological effects of epicatechin (ECN), a crosslinking agent, on human dental pulp cells (hDPCs) cultured in collagen scaffolds. Material and Method To evaluate the effects of ECN on the proliferation of hDPCs, cell counting was performed using optical and fluorescent microscopy. Measurements of alkaline phosphatase (ALP) activity, alizarin red staining, and real-time polymerase chain reactions were performed to assess odontogenic differentiation. The compressive strength and setting time of collagen scaffolds containing ECN were measured. Differential scanning calorimetry was performed to analyze the thermal behavior of collagen in the presence of ECN. Results Epicatechin increased ALP activity, mineralized nodule formation, and the mRNA expression of dentin sialophosphoprotein (DSPP), a specific odontogenic-related marker. Furthermore, ECN upregulated the expression of DSPP in hDPCs cultured in collagen scaffolds. Epicatechin activated the extracellular signal-regulated kinase (ERK) and the treatment with an ERK inhibitor (U0126) blocked the expression of DSPP. The compressive strength was increased and the setting time was shortened in a dose-dependent manner. The number of cells cultured in the ECN-treated collagen scaffolds was significantly increased compared to the cells in the untreated control group. Conclusions Our results revealed that ECN promoted the proliferation and differentiation of hDPCs. Furthermore, the differentiation was regulated by the ERK signaling pathway. Changes in mechanical properties are related to cell fate, including proliferation and differentiation. Therefore, our study suggests the ECN treatment might be desirable for dentin-pulp complex regeneration.
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Full text: Available Index: LILACS (Americas) Main subject: Catechin / Collagen / Cross-Linking Reagents / Dental Pulp / Tissue Scaffolds Type of study: Evaluation studies Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: South Korea Institution/Affiliation country: Chonbuk National University/KR

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Full text: Available Index: LILACS (Americas) Main subject: Catechin / Collagen / Cross-Linking Reagents / Dental Pulp / Tissue Scaffolds Type of study: Evaluation studies Limits: Humans Language: English Journal: J. appl. oral sci Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: South Korea Institution/Affiliation country: Chonbuk National University/KR