Assessment of Epstein-Barr virus nucleic acids in gastric but not in breast cancer by next-generation sequencing of pooled Mexican samples
Mem. Inst. Oswaldo Cruz
;
111(3): 200-208, Mar. 2016. tab, graf
Article
in English
| LILACS
| ID: lil-777367
ABSTRACT
Gastric (GC) and breast (BrC) cancer are two of the most common and deadly tumours. Different lines of evidence suggest a possible causative role of viral infections for both GC and BrC. Wide genome sequencing (WGS) technologies allow searching for viral agents in tissues of patients with cancer. These technologies have already contributed to establish virus-cancer associations as well as to discovery new tumour viruses. The objective of this study was to document possible associations of viral infection with GC and BrC in Mexican patients. In order to gain idea about cost effective conditions of experimental sequencing, we first carried out an in silico simulation of WGS. The next-generation-platform IlluminaGallx was then used to sequence GC and BrC tumour samples. While we did not find viral sequences in tissues from BrC patients, multiple reads matching Epstein-Barr virus (EBV) sequences were found in GC tissues. An end-point polymerase chain reaction confirmed an enrichment of EBV sequences in one of the GC samples sequenced, validating the next-generation sequencing-bioinformatics pipeline.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Stomach Neoplasms
/
Breast Neoplasms
/
DNA, Viral
/
RNA, Viral
/
High-Throughput Nucleotide Sequencing
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Mexico
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2016
Type:
Article
/
Project document
Affiliation country:
Mexico
Institution/Affiliation country:
Hospital Infantil de México Federico Gómez/MX
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