Monoclonal antibodies generated against activated B-cells: unexpected specific reactivity to T-cells

Moura, A. C. N; Moreira Filho, C. A; Hartmann, K. U

Moura, A. C. N; Moreira Filho, C. A; Hartmann, K. U.

Braz. j. med. biol. res ; 22(8): 983-6, 1989. tab

Article in English | LILACS | ID: lil-77738

RESUMO

RESUMO

Monoclonal antibodies (Mabs) were produced aginst (C57B1/6 "nude" lymphoblast cells. They are IgG2a and do not react with mouse serum proteins. Five Mabs were characterixed functional by immunohistochemistry and immunofluorescence in spleen, lymph nodes and Peyer's patches from nude and normal mice, an anormal mouse thymus. Two Mabs reacted with containing B-cells as indicared by immunoperoxidase staining, another recognized T- and B-cell sites and two specifically stained T lymphocyte-containing areas. Immunofluorescence studies confirmed the sites identified above by immunohistochemistry

Subject(s)

Mice; Rats; Animals; Antibodies, Monoclonal/biosynthesis; B-Lymphocytes/immunology; T-Lymphocytes/immunology; Cloning, Molecular; Fluorescent Antibody Technique; Lymphocyte Activation

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Index: LILACS (Americas) Main subject: B-Lymphocytes / T-Lymphocytes / Antibodies, Monoclonal Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 1989 Type: Article / Congress and conference / Project document

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