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Effect of irradiation with different laser wavelengths on oxidative stress of non-hepatectomized rats
Mumic, Fabricia Teixeira; Silveira, Marina Rodrigues Garcia da; Vilalva, Kelvin Henrique; Jordani, Maria Eliza; Gomes, Maria Cecilia Jordani; Vanni, José Carlos; Vollet Filho, José Dirceu; Kurachi, Cristina; Bagnato, Vanderlei Salvador; Silva, Orlando de Castro e.
  • Mumic, Fabricia Teixeira; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Silveira, Marina Rodrigues Garcia da; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Vilalva, Kelvin Henrique; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Jordani, Maria Eliza; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Gomes, Maria Cecilia Jordani; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Vanni, José Carlos; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Vollet Filho, José Dirceu; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Kurachi, Cristina; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Bagnato, Vanderlei Salvador; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
  • Silva, Orlando de Castro e; University of São Paulo. Ribeirão Preto Medical School. Ribeirão Preto. BR
Acta cir. bras ; 31(supl.1): 40-44, 2016. graf
Article in English | LILACS | ID: lil-779759
ABSTRACT

PURPOSE:

To assess the effect of two laser wavelengths, either separate or combined, on intact rat livers.

METHOD:

Nineteen male Wistar rats (200-300 g) were submitted to laser irradiation at 5 different sites on the liver surface.Wavelengths 660 and 780 nm were used, with a dose of irradiation of 60 J/cm2/site.The animals were divided into the groupscontrol (C) and animals irradiated with 660 nm laser (L1), with 780 nm laser (L2) or withboth wavelengths (L3).Mitochondrial function, mitochondrial swelling, and hepatocellular malondialdehyde (MDA) levels were determined.Data were analyzed by the Mann-Whitney test, with the level of significance set at 5%.

RESULTS:

There was a reduction of ADP-activated respiration (state 3) in group L1 compared to group C (p=0.0016), whereas the values of group L2 were similar to control.Group L3 also showed a reduction of state 3 (p=0.0159).There was a reduction of RCR in group L1 compared to control (p=0.0001) and to group L2 (p=0.0040).Mitochondrial swelling only differed between group L3 and control (p=0.0286).There was a increase in MDA levels in group L3 compared to control (p=0.0476) and to group L2 (p=0.0286) and in group L1 compared to group L2 (p=0.0132).

CONCLUSION:

Although laser irradiation reduced mitochondrial function,it did not interfere with the hepatocellular energy status.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Mitochondria, Liver / Oxidative Stress / Lasers, Semiconductor / Liver Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Mitochondria, Liver / Oxidative Stress / Lasers, Semiconductor / Liver Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2016 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR