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Guanidinoacetate alters antioxidant defenses and butyrylcholinesterase activity in the blood of rats
Eger, Guilherme André; Ferreira, Vinícius Vialle; Batista, Camila Ribeiro; Lima, Daniela Delwing de; Wyse, Angela Terezinha de Souza; Cruz, Júlia Niehues da; Magro, Débora Delwing Dal; Cruz, José Geraldo Pereira da.
  • Eger, Guilherme André; Regional University of Blumenau. Department of Medicine. Blumenau. BR
  • Ferreira, Vinícius Vialle; Regional University of Blumenau. Department of Medicine. Blumenau. BR
  • Batista, Camila Ribeiro; Regional University of Blumenau. Department of Medicine. Blumenau. BR
  • Lima, Daniela Delwing de; University of Joinville Region. Department of Medicine. Joinvile. BR
  • Wyse, Angela Terezinha de Souza; Federal University of Rio Grande do Sul. Department of Biochemistry. Porto Alegre. BR
  • Cruz, Júlia Niehues da; Hans Dieter Schmidt Hospital. Joinville. BR
  • Magro, Débora Delwing Dal; Regional University of Blumenau. Department of Natural Sciences. Blumenau. BR
  • Cruz, José Geraldo Pereira da; Regional University of Blumenau. Department of Natural Sciences. Blumenau. BR
Clin. biomed. res ; 35(1): 49-54, 2015. ilus
Article in English | LILACS | ID: lil-780276
ABSTRACT
Deficiency of guanidinoacetate methyltransferase, the first described creatine biosynthesis defect, leads to depletion of creatine and phosphocreatine, and accumulation of guanidinoacetate (GAA) in brain and body fluids. The present study aimed to investigate the influence of GAA on the activities of antioxidant enzymes, as well as on thiobarbituric acid-reactive substances (TBARS) and butyrylcholinesterase (BuChE) activity in the blood of rats. We also evaluated the effect of trolox (6-hydr oxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), GSH (glutathione) and L-NAME (NG-nitro-L-arginine methyl ester) on the alterations elicited by GAA.

Methods:

The rats were randomly divided into 8 groups (1) control; (2) GAA (10, 30, 50, 100 mM/kg); (3) trolox (1 mM/kg) + control; (4) trolox (1 mM/kg) + GAA (100 mM/kg); (5) GSH (1 mM/kg) + control; (6) GSH (1 mM/kg) + GAA (100 mM/kg); (7) L-NAME (1 mM/kg) + control; (8) L-NAME + GAA (100 mM/kg). After the addition of compounds, erythrocytes and plasma were pre-incubated at 37°C for 1h and tested immediately.

Results:

GAA enhanced the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in the erythrocytes and BuChE activity. In addition, GAA enhanced TBARS levels in the plasma. Trolox, GSH and L-NAME addition prevented the majority of alterations in oxidative stress parameters and the increase of BuChE activity that were caused by GAA. Data suggest that GAA alters antioxidant defenses and induces lipid peroxidation in the blood, as well altering BuChE activity. However, in the presence of trolox, GSH and L-NAME some of these alterations in oxidative stress and BuChE activity were prevented.

Conclusions:

Our findings lend support to a potential therapeutic strategy for this condition, which may include the use of appropriate antioxidants for ameliorating the damage caused by GAA...
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Butyrylcholinesterase / Oxidative Stress / Guanidinoacetate N-Methyltransferase / Antioxidants Limits: Animals Language: English Journal: Clin. biomed. res Journal subject: Medicine Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Rio Grande do Sul/BR / Hans Dieter Schmidt Hospital/BR / Regional University of Blumenau/BR / University of Joinville Region/BR

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Full text: Available Index: LILACS (Americas) Main subject: Butyrylcholinesterase / Oxidative Stress / Guanidinoacetate N-Methyltransferase / Antioxidants Limits: Animals Language: English Journal: Clin. biomed. res Journal subject: Medicine Year: 2015 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Rio Grande do Sul/BR / Hans Dieter Schmidt Hospital/BR / Regional University of Blumenau/BR / University of Joinville Region/BR