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Occurrence and characteristics of extended spectrum beta-lactamases-producing Enterobacteriaceae from foods of animal origin
Tekiner, İsmail Hakkı; Özpınar, Haydar.
  • Tekiner, İsmail Hakkı; Istanbul Aydın University. Department of Food Engineering. Istanbul. TR
  • Özpınar, Haydar; Istanbul Aydın University. Department of Food Engineering. Istanbul. TR
Braz. j. microbiol ; 47(2): 444-451, Apr.-June 2016. tab
Article in English | LILACS | ID: lil-780833
ABSTRACT
Abstract Presence of extended spectrum beta-lactamases (ESBL) in bacteria is a growing health concern of global significance. The local, regional, national, and international epidemiological studies for extended spectrum beta-lactamases-producing Enterobacteriaceae and their encoding genes in foods are still incomplete. The objective of this study was to determine the occurrence of extended spectrum beta-lactamases-producing Enterobacteriaceae and the characteristics of their encoding genes from a total of 250 samples of various foods of animal-origin (100 raw chicken meat, 100 raw cow milk, and 50 raw cow milk cheese) sold in Turkey. Overall, 55 isolates were positive as extended spectrum beta-lactamases-producing Enterobacteriaceae. The most prevalent extended spectrum beta-lactamases-producing strain were identified as Escherichia coli (80%), followed by Enterobacter cloacae (9.1%), Citrobacter braakii (5.5%), Klebsiella pneumoniae (3.6%), and Citrobacter werkmanii (1.8%) by Vitek® MS. The simultaneous production of extended spectrum beta-lactamases and AmpC was detected in five isolates (9.1%) in E. coli (80%) and E. cloacae (20%). The frequency rates of blaTEM, blaCTX-M, and blaSHV were 96.4%, 53.7%, and 34.5%, respectively. The co-existence of bla -genes was observed in 82% of extended spectrum beta-lactamases producers with a distribution of blaTEM & blaCTX-M (52.7%), blaTEM & blaSHV (20%), blaTEM & blaCTX-M & blaSHV (12.7%), and blaSHV & blaCTX-M (1.8%). The most prevalent variant of blaCTX-M clusters was defined as blaCTX-M-1 (97.2%), followed by blaCTX-M-8 (2.8%). In summary, the analysed foods were found to be posing a health risk for Turkish consumers due to contamination by Enterobacteriaceae with a diversity of extended spectrum beta-lactamases encoding genes.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Bacterial Proteins / Beta-Lactamases / Cheese / Milk / Enterobacteriaceae / Meat Type of study: Prognostic study Country/Region as subject: Asia Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2016 Type: Article Affiliation country: Turkey Institution/Affiliation country: Istanbul Aydın University/TR

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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Proteins / Beta-Lactamases / Cheese / Milk / Enterobacteriaceae / Meat Type of study: Prognostic study Country/Region as subject: Asia Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2016 Type: Article Affiliation country: Turkey Institution/Affiliation country: Istanbul Aydın University/TR