Low impact to fixed cell processing aiming transmission electron microscopy

Barth, Ortrud Monika; Silva, Marcos Alexandre Nunes da; Barreto-Vieira, Debora Ferreira

Barth, Ortrud Monika; Silva, Marcos Alexandre Nunes da; Barreto-Vieira, Debora Ferreira.

Barth, Ortrud Monika; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro. BR
Silva, Marcos Alexandre Nunes da; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro. BR
Barreto-Vieira, Debora Ferreira; Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro. BR

Mem. Inst. Oswaldo Cruz ; 111(6): 411-413, June 2016. graf

Article in English | LILACS | ID: lil-784251

ABSTRACT

ABSTRACT

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.

Subject(s)

Animals; Aedes/ultrastructure; Dengue Virus/ultrastructure; Microscopy, Electron, Transmission/methods; Aedes/virology; Cell Culture Techniques; Centrifugation/methods; Chlorocebus aethiops; Fixatives; Indicators and Reagents; Vero Cells/ultrastructure

Cell fixation; DENV; Low impact; Transmission electron microscopy

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Full text: Available Index: LILACS (Americas) Main subject: Aedes / Dengue Virus / Microscopy, Electron, Transmission Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2016 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR

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