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Neutrophil elastase ameliorates matrix metalloproteinase-9 to promote lipopolysaccharide-induced acute lung injury in mice 1
Guo, Yingfei; Ma, Lingyun; Zhang, Fei; Sun, Rongju; Li, Tanshi.
  • Guo, Yingfei; General Hospital of PLA. Department of Emergency. Beijing. CN
  • Ma, Lingyun; General Hospital of PLA. Department of Emergency. Beijing. CN
  • Zhang, Fei; General Hospital of PLA. Department of Emergency. Beijing. CN
  • Sun, Rongju; General Hospital of PLA. Department of Emergency. Beijing. CN
  • Li, Tanshi; General Hospital of PLA. Department of Emergency. Beijing. CN
Acta cir. bras ; 31(6): 382-388, graf
Article in English | LILACS | ID: lil-785018
ABSTRACT
ABSTRACT

PURPOSE:

To investigate the regulatory roles of neutrophil elastase (NE) and matrix metalloproteinase-9 (MMP-9) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice.

METHODS:

To construct LPS-induced ALI mouse models, wild-type C57BL/6 mice were administered 5.0 mg/kg of LPS through endotracheal, and/or 1.0 mg/kg of ONO-5046, and/or 20.0 mg/kg of chemically modified tetracycline-3 (CMT-3) by gavage. The levels of MMP-9, tissue inhibitor of metalloprotease-1, interleukin (IL)-6 were detected by real time RT-PCR at 6 h, 24 h and 48 h, and tumor necrosis factor (TNF), lung wet-dry weight ratio, white blood cell (WBC) count and polymorphonuclear (PMN) count in bronchoalveolar lavage fluid (BALF) were tested at 48 h after administration. The 5-day survival analysis of the ALI mice was also performed.

RESULTS:

Both ONO-5046 and CMT-3, regardless of being used individually or combined, significantly reduced the levels of MMP-9, IL-6, and TNF in lung tissue as well as in BALF, and the WBC and PMN count in BALF. Combined treatment with ONO-5046 and CMT-3 remarkably improved the survival rate of ALI mice.

CONCLUSION:

Neutrophil elastase synergizes with matrix metalloproteinase-9 to promote and regulate the release of inflammatory mediators and the infiltration of inflammatory cells, consequently affecting the survival of lipopolysaccharide-induced acute lung injury mice.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Sulfonamides / Tetracyclines / Leukocyte Elastase / Matrix Metalloproteinase 9 / Acute Lung Injury / Glycine Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2016 Type: Article Affiliation country: China Institution/Affiliation country: General Hospital of PLA/CN

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Full text: Available Index: LILACS (Americas) Main subject: Sulfonamides / Tetracyclines / Leukocyte Elastase / Matrix Metalloproteinase 9 / Acute Lung Injury / Glycine Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2016 Type: Article Affiliation country: China Institution/Affiliation country: General Hospital of PLA/CN