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Effects of melatonin on tibia bone defects in rats / Efectos de la melatonina sobre defectos óseos en la tibia de ratas
Koparal, M; Irtegün, S; Alan, H; Deveci, E; Gülsün, B; Pektanç, G.
  • Koparal, M; Adiyaman University. Faculty of Dentistry. Department of Oral and Maxillofacial Surgery. Adiyaman. TR
  • Irtegün, S; University of Dicle. Faculty of Medicine. Department of Medical Biology. Diyarbakir. TR
  • Alan, H; Inonu University. Faculty of Dentistry. Department of Oral and Maxillofacial Surgery. Malatya. TR
  • Deveci, E; University of Dicle. Faculty of Medicine. Department of Histology and Embryology. Diyarbakir. TR
  • Gülsün, B; Dicle University. Faculty of Dentistry. Department of Oral and Maxillofacial Surgery. Diyarbakir. TR
  • Pektanç, G; University of Dicle. Faculty of Medicine. Department of Histology and Embryology. Diyarbakir. TR
Int. j. morphol ; 34(2): 763-769, June 2016. ilus
Article in English | LILACS | ID: lil-787066
Responsible library: CL1.1
ABSTRACT
The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length ) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone formation and myeloid tissue in bone defects. Immunohistochemical and immunoblot analysis demonstrated non-collagenous proteins (osteopontin and osteonectin) differences in tibial bone defects. The expression of osteopontin on tibia was increased by 14 days melatonin treatment. The expression of osteonectin on tibia was dramatically increased by 14 days melatonin treatment.
RESUMEN
El objetivo fue evaluar por medio de análisis histopatológico e inmunohistoquímico los efectos cicatrizantes de la melatonina en un modelo de defecto óseo tibial en ratas. Se utilizaron 21 ratas albinas Wistar macho. En cada animal, se crearon defectos óseos en las tibias de 6 mm de longitud. Los animales se dividieron en tres grupos. El Grupo 1 correspondió al grupo control (defectos tibiales sin tratamiento). Al Grupo 2 se administró melatonina por vía intraperitoneal (10 mg/kg) 14 días posteriores al defecto tibial. Al Grupo 3 se administró melatonina por vía intraperitoneal (10 mg/kg) 28 días posteriores al defecto tibial. Se realizó un análisis histopatológico para evaluar los procesos de actividad osteoblástica, formación de matriz, formación de hueso trabecular y tejido mieloide en los defectos óseos. Los análisis inmunohistoquímicos y de inmunotransferencia mostraron diferencias de proteínas no colágenas (osteopontina y osteonectina). La expresión de osteopontina en defectos óseos tibiales se incrementó en el Grupo 2. La expresión de osteonectina en la tibia se incrementó fuertemente bajo el tratamiento con melatonina por 14 días.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Tibia / Tibial Fractures / Melatonin Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2016 Type: Article Affiliation country: Turkey Institution/Affiliation country: Adiyaman University/TR / Dicle University/TR / Inonu University/TR / University of Dicle/TR

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Full text: Available Index: LILACS (Americas) Main subject: Tibia / Tibial Fractures / Melatonin Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2016 Type: Article Affiliation country: Turkey Institution/Affiliation country: Adiyaman University/TR / Dicle University/TR / Inonu University/TR / University of Dicle/TR