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Pharmacological study of the mechanisms involved in the vasodilator effect produced by the acute application of triiodothyronine to rat aortic rings
Lozano-Cuenca, J; López-Canales, O A; Aguilar-Carrasco, J C; Villagrana-Zesati, J R; López-Mayorga, R M; Castillo-Henkel, E F; López-Canales, J S.
  • Lozano-Cuenca, J; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
  • López-Canales, O A; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
  • Aguilar-Carrasco, J C; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
  • Villagrana-Zesati, J R; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
  • López-Mayorga, R M; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
  • Castillo-Henkel, E F; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
  • López-Canales, J S; National Institute of Perinatology. Department of Cellular Biology. Mexico City. MX
Braz. j. med. biol. res ; 49(8): e5304, 2016. graf
Article in English | LILACS | ID: lil-787383
ABSTRACT
A relationship between thyroid hormones and the cardiovascular system has been well established in the literature. The present in vitro study aimed to investigate the mechanisms involved in the vasodilator effect produced by the acute application of 10-8–10-4 M triiodothyronine (T3) to isolated rat aortic rings. Thoracic aortic rings from 80 adult male Wistar rats were isolated and mounted in tissue chambers filled with Krebs-Henseleit bicarbonate buffer in order to analyze the influence of endothelial tissue, inhibitors and blockers on the vascular effect produced by T3. T3 induced a vasorelaxant response in phenylephrine-precontracted rat aortic rings at higher concentrations (10-4.5–10-4.0 M). This outcome was unaffected by 3.1×10-7 M glibenclamide, 10-3 M 4-aminopyridine (4-AP), 10-5 M indomethacin, or 10-5 M cycloheximide. Contrarily, vasorelaxant responses to T3 were significantly (P<0.05) attenuated by endothelium removal or the application of 10-6 M atropine, 10-5 M L-NG-nitroarginine methyl ester (L-NAME), 10-7 M 1H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), 10-6 M (9S,10R,12R)-2,3,9,10,11,12-Hexahydro-10-methoxy-2,9-dimethyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg3′,2′,1′-kl]pyrrolo[3,4-i](1,6)benzodiazocine-10-carboxylic acid, methyl ester KT 5823, 10-2 M tetraethylammonium (TEA), or 10-7 M apamin plus 10-7 M charybdotoxin. The results suggest the involvement of endothelial mechanisms in the vasodilator effect produced by the acute in vitro application of T3 to rat aortic rings. Possible mechanisms include the stimulation of muscarinic receptors, activation of the NO-cGMP-PKG pathway, and opening of Ca2+-activated K+ channels.
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Full text: Available Index: LILACS (Americas) Main subject: Aorta, Thoracic / Triiodothyronine / Vasodilation / Vasodilator Agents / Endothelium, Vascular Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: Mexico Institution/Affiliation country: National Institute of Perinatology/MX

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Full text: Available Index: LILACS (Americas) Main subject: Aorta, Thoracic / Triiodothyronine / Vasodilation / Vasodilator Agents / Endothelium, Vascular Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article Affiliation country: Mexico Institution/Affiliation country: National Institute of Perinatology/MX