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Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants
Pavan, T R; Di Domenico, J; Kirsten, K S; Nied, C O; Frandoloso, R; Kreutz, L C.
  • Pavan, T R; Universidade de Passo Fundo. Programa de Pós-Graduação em Bioexperimentação. Passo Fundo. BR
  • Di Domenico, J; Universidade de Passo Fundo. Programa de Pós-Graduação em Bioexperimentação. Passo Fundo. BR
  • Kirsten, K S; Universidade de Passo Fundo. Programa de Pós-Graduação em Bioexperimentação. Passo Fundo. BR
  • Nied, C O; Universidade de Passo Fundo. Programa de Pós-Graduação em Bioexperimentação. Passo Fundo. BR
  • Frandoloso, R; Universidade de Passo Fundo. Programa de Pós-Graduação em Bioexperimentação. Passo Fundo. BR
  • Kreutz, L C; Universidade de Passo Fundo. Programa de Pós-Graduação em Bioexperimentação. Passo Fundo. BR
Braz. j. med. biol. res ; 49(8): e5281, 2016. graf
Article in English | LILACS | ID: lil-787384
ABSTRACT
Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species.
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Full text: Available Index: LILACS (Americas) Main subject: Catfishes / Adjuvants, Immunologic / Vaccination / Antibody Formation Type of study: Risk factors Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de Passo Fundo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Catfishes / Adjuvants, Immunologic / Vaccination / Antibody Formation Type of study: Risk factors Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2016 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de Passo Fundo/BR