The prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in Inner Mongolia, China
Braz. j. microbiol
;
47(3): 691-696, July-Sept. 2016. tab
Article
in English
| LILACS
| ID: lil-788958
ABSTRACT
ABSTRACT This study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in Inner Mongolia, China. Screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. Out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding genes and virulence determinants using polymerase chain reaction -based methods. Enterococcus faecium and Enterococcus faecalis were identified as the species of greatest clinical importance. The aac(6')-Ie-aph(2")-Ia and ant(6')-Ia genes were found to be the most common aminoglycoside-modifying enzyme genes among high-level gentamicin resistance and high-level streptomycin resistance isolates, respectively. Moreover, gelE was the most common virulence gene among high-level aminoglycoside resistance isolates. Compared to Enterococcus faecium, Enterococcus faecalis harbored multiple virulence determinants. The results further indicated no correlation between aminoglycoside-modifying enzyme gene profiles and the distribution of virulence genes among the enterococcal isolates with high-level gentamicin resistance or high-level streptomycin resistance evaluated in our study.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Gene Expression Regulation, Bacterial
/
Gene Expression Regulation, Enzymologic
/
Enterococcus
/
Drug Resistance, Bacterial
/
Aminoglycosides
Type of study:
Prevalence study
/
Risk factors
Country/Region as subject:
Asia
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2016
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Baotou Medical College/CN
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