Simultaneous production of amylases and proteases by Bacillus subtilis in brewery wastes
Braz. j. microbiol
;
47(3): 665-674, July-Sept. 2016. tab, graf
Article
in English
| LILACS
| ID: lil-788963
ABSTRACT
ABSTRACT The simultaneous production of amylase (AA) and protease (PA) activity by Bacillus subtilis UO-01 in brewery wastes was studied by combining the response surface methodology with the kinetic study of the process. The optimum conditions (T = 36.0 °C and pH = 6.8) for high biomass production (0.92 g/L) were similar to the conditions (T = 36.8 °C and pH = 6.6) for high AA synthesis (9.26 EU/mL). However, the maximum PA level (9.77 EU/mL) was obtained at pH 7.1 and 37.8 °C. Under these conditions, a considerably high reduction (between 69.9 and 77.8%) of the initial chemical oxygen demand of the waste was achieved. In verification experiments under the optimized conditions for production of each enzyme, the AA and PA obtained after 15 h of incubation were, respectively, 9.35 and 9.87 EU/mL. By using the Luedeking and Piret model, both enzymes were classified as growth-associated metabolites. Protease production delay seemed to be related to the consumption of non-protein and protein nitrogen. These results indicate that the brewery waste could be successfully used for a high scale production of amylases and proteases at a low cost.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Peptide Hydrolases
/
Bacillus subtilis
/
Fermentation
/
Amylases
/
Industrial Waste
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2016
Type:
Article
Affiliation country:
Cuba
Institution/Affiliation country:
Universidad de Oriente/CU
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