Efecto del factor de crecimiento de fibroblastos 21 / Fibroblast growht factor 21 effects over phosphatase SHP-2 expression in Malme-3 cells
Rev. chil. endocrinol. diabetes
;
7(3): 99-103, jul.2014. graf
Article
in Spanish
| LILACS
| ID: lil-789305
ABSTRACT
Almost 50 percent of Noonan syndrome patients, characterized by short stature, present activating mutations of the citoplasmatic phosphatase SHP-2, which induce hyperactivation of the Ras/MAPK pathway. On the other hand, the fibroblast growth factor 21 (FGF-21), recently suggested as a FGF with endocrine function, would affect longitudinal growth inhibiting growth hormone signaling at chondrocytes level. Union and activation of FGF-21 to its receptor is regulated by the co-factor beta Klotho (KLB). Aims:
To determine if FGF-21 and/or FGF-21+KLB are able to modify the genetic expression of SHP-2 ina human skin fibroblast cell line (Malme-3).Methods:
cells were incubated with or without FGF-21, FGF-21 + KLB. At 12 and 24 hours after induction total RNA was extracted andSHP-2 mRNA levels were determine by quantitative PCR. Expression of GADPH gene was employed for normalization.Results:
Incubation with FGF-21 produce a 36 percent (p = < 0,05)increment in SHP-2 expression, which was not modified with KLB co-incubation.Discussion:
it is shown by the first time that FGF-21 is able to produce an increase in SHP-2 gene expression in human fibroblast, which was independent of KLB presence...
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Fibroblast Growth Factors
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. chil. endocrinol. diabetes
Journal subject:
Endocrinology
Year:
2014
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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