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A modified method by differential adhesion for enrichment of bladder cancer stem cells
Zhu, Yong-tong; Pang, Shi-yu; Luo, Yang; Chen, Wei; Bao, Ji-ming; Tan, Wan-long.
  • Zhu, Yong-tong; Southern Medical University. Nanfang Hospital. Department of Obstetrics and Gynecology, Center for Reproductive Medicine. Guangzhou. CN
  • Pang, Shi-yu; Southern Medical University. Nanfang Hospital. Department of Obstetrics and Gynecology, Center for Reproductive Medicine. Guangzhou. CN
  • Luo, Yang; Southern Medical University. Nanfang Hospital. Department of Obstetrics and Gynecology, Center for Reproductive Medicine. Guangzhou. CN
  • Chen, Wei; Southern Medical University. Nanfang Hospital. Department of Obstetrics and Gynecology, Center for Reproductive Medicine. Guangzhou. CN
  • Bao, Ji-ming; Southern Medical University. Nanfang Hospital. Department of Obstetrics and Gynecology, Center for Reproductive Medicine. Guangzhou. CN
  • Tan, Wan-long; Southern Medical University. Nanfang Hospital. Department of Obstetrics and Gynecology, Center for Reproductive Medicine. Guangzhou. CN
Int. braz. j. urol ; 42(4): 817-824, July-Aug. 2016. tab, graf
Article in English | LILACS | ID: lil-794669
Responsible library: BR1.1
ABSTRACT
ABSTRACT

Purpose:

In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials and

Methods:

Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay.

Results:

Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo.

Conclusion:

Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Neoplastic Stem Cells / Urinary Bladder Neoplasms / Trypsin / Cell Adhesion / Cell Separation / Cell Culture Techniques Limits: Animals Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2016 Type: Article Affiliation country: China Institution/Affiliation country: Southern Medical University/CN

Full text: Available Index: LILACS (Americas) Main subject: Neoplastic Stem Cells / Urinary Bladder Neoplasms / Trypsin / Cell Adhesion / Cell Separation / Cell Culture Techniques Limits: Animals Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2016 Type: Article Affiliation country: China Institution/Affiliation country: Southern Medical University/CN