Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification
Braz. arch. biol. technol
;
59(spe): e16160500, 2016. tab, graf
Article
in English
| LILACS
| ID: lil-796855
ABSTRACT
ABSTRACT A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 oC and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the addition of fluorescent dye. The detection limit of the LAMP assay was approximately 6 copies of the target gene per microliter, 100 times more sensitive than conventional PCR. LAMP is a simple and inexpensive molecular assay format for ASFV detection. To date, African swine fever has not been reported in China. LAMP can be used to monitor ASFV spread into China, thereby reducing the threat of ASF.
Full text:
Available
Index:
LILACS (Americas)
Type of study:
Diagnostic study
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2016
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Sichuan Agricultural University/CN
Similar
MEDLINE
...
LILACS
LIS