Restriction endonucleases from microorganisms isolated in Brazil: an isoschizomer of Hae III from a thermophilic Bacillus SP
Braz. j. med. biol. res
;
22(11): 1321-8, 1989. ilus
Article
in English
| LILACS
| ID: lil-82989
RESUMO
The isolation and characterization of a restriction endonuclease from a thermophilic strain of Bacillus is described. The enzyme recognizes the palindromic sequence 5'...GGCC...3' as determined by PEI-cellulose chromatography of pancreativc DNAse and snake venom phosphodieterase digestion products of labelled DNA fragments, analysis of restriction digests and direct sequence analysis. The enzyme, denominated BspBR, is an isoschizomer of HaeIII and BspRI
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Index:
LILACS (Americas)
Main subject:
Bacillus
/
DNA Restriction Enzymes
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1989
Type:
Article
/
Project document
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