A technical improvement for the thyroid peroxidase iodination assay
Braz. j. med. biol. res
;
22(7): 821-3, 1989. ilus
Article
in English
| LILACS
| ID: lil-83198
RESUMO
Thyroid peroxidase (TPO) iodination activity is generally evaluated in vitro by the iodination of poorly iodinated thyroglobulin or bovine serum albumin, followed by separation of protein-bound and inorganic iodide by paper chromatography,. Precipitation of protein-bound iodine by trichloroacetic acid (TCA) was evaluated as an alternative to the time-consuming paper chromatographic separation (PC) in normal rat TPO preparations. The protein-bound iodine estimates as well as the iodination activities determined by these two procedures were significantly correlated (r = 0.95 and 0.98, respectively, P<0.001), and the iodination activities regression line slope (b = 0.97 + or - 0.11) was not different from 1. The protein-bound iodine separation by TCA is simpler and faster, without loss of precision. Thus, it can be a useful alternative step in the thyroid peroxidase iodination assay
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Index:
LILACS (Americas)
Main subject:
Thyroid Gland
/
In Vitro Techniques
/
Iodide Peroxidase
/
Iodine
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1989
Type:
Article
/
Congress and conference
/
Project document
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