Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
Mem. Inst. Oswaldo Cruz
;
85(1): 107-11, jan.-mar. 1990. tab, ilus
Article
in English
| LILACS
| ID: lil-85177
RESUMO
Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacterial Outer Membrane Proteins
/
Yersinia pestis
/
Cell Membrane
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1990
Type:
Article
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