Structural studies on allergen RC-13 from Ricinus communis L.: isolation and characterization of a major glycopeptide
An. acad. bras. ciênc
;
62(1): 17-24, mar. 1990. tab
Article
in English
| LILACS
| ID: lil-92236
RESUMO
A glycoprotein, RC-13, isolated from Ricinus communis seeds was reduced, S-alkylated and cleaved by trypsin. The tryptic digest was fractionated by ion-exchange chromatography and a glycopeptide was isolated and purified by high-voltage paper electrophoresis. When submitted to amino acid and carbohydrate analyses this major glycopeptide showed the following chemical composition: Lys1, Asp1, Thr2, Ser4, Glu1, Pro2, Gly2, Ala2, Val2, GlcN6, Man6 and Gal8. Hydrazynolysis positioned Ser as the C-terminal residue. It is postulated that this glycopeptide belongs to the C-terminal region of the allergen
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Index:
LILACS (Americas)
Main subject:
Seeds
/
Allergens
/
Glycopeptides
/
Ricinus communis
/
Amino Acids
Language:
English
Journal:
An. acad. bras. ciênc
Journal subject:
Science
Year:
1990
Type:
Article
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