Use of y-chromosome-specific DNA probes to evaluate an XX male
Braz. j. med. biol. res
;
24(2): 149-56, 1991. tab
Article
in English
| LILACS
| ID: lil-99449
ABSTRACT
The function of a Y human chromosomal DNA sequence was evaluated. The Y-5 probe was isolated from a flow-sorted chromosome library and detects Y-specific sequences. The Y-5 probe and other T-specific probes were used to analyze an XX male patient without ambiguous genitalia. DNA sequences from the short arm of the chromosome Y that were detected with pDP1007 and DP105 in the patient's genome explain the testis differentation observed in this case. Failure of the patient's DNA to hybridize to the Y-5 probe shows that the primitive gonads can differentiate into testes even in the absence of this chromosome region. In contrast, a gene controlling spermatogenesis may exist in this region because the patient azoospermic
Search on Google
Index:
LILACS (Americas)
Main subject:
Sex Chromosome Aberrations
/
Sex Determination Analysis
/
Y Chromosome
/
DNA Probes
Limits:
Adolescent
/
Humans
/
Male
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1991
Type:
Article
/
Congress and conference
Similar
MEDLINE
...
LILACS
LIS