Expression of an active foot-and-mouth disease virus RNA polymerase in Escherichia coli
Braz. j. med. biol. res
;
24(4): 359-63, 1991. ilus
Article
in English
| LILACS
| ID: lil-99464
ABSTRACT
The expression of a native form of the foot-and-mouth disease virus RNA polymerase was obtained. Two oligonucleotides of 66 base pairs were used to renuild the 5' end of the gene and to introduce the first methionine codon. The expression of the active polymerase in E. coli was achieved by inserting the gene before the tac promoter of the pKK223-3 plasmid
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Index:
LILACS (Americas)
Main subject:
DNA-Directed RNA Polymerases
/
Gene Expression Regulation, Viral
/
Escherichia coli
/
Hand, Foot and Mouth Disease
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1991
Type:
Article
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