Functional and ultrastructural evaluation of myocardial protection provided by intermittent coronary sinus perfusion in the isolated dog heart
Braz. j. med. biol. res
;
24(1): 81-91, jan.-mar. 1991. tab
Article
in English
| LILACS
| ID: lil-99585
RESUMO
The protection offered by intermittent perfusion of cardioplegic solution through the coronary sinus was investigated in isovolumic blood-perfused dog heart preparations submitted to 60 min of ischemia and 45 min of reperfusion. The prepations were divided into three treatment groups a) coronary sinus, consisting of preparations (N=10) perfused through the coronary sinus under 40 cm water pressure b) aortic, consisting of preparations (N=10) perfused through the aortic stump under 100 mm Hg pressure; c) control,consisting of hearts (N=9) that were not perfused with cardioplegic solution. Properties of contractile capacity and relaxation were markedly impaired in the control group but were preserved to a comparable extent in the groups perfused with cardioplegic solution through the aorta and coronary sinus. Developed pressure decreased in the control group (before ischemia 70 ñ 5.5 mmHg; after reperfusion 35 ñ 12 mmHg; P,0.05) and didn't vary in the aortic group (from 69 ñ 4mmHg to 65 ñ 13 mmHg P>0.05) and coronary sinus group (from 69 ñ 4.6 mmHg to 60 ñ 10 mmHg; P>0.05). Myocardial relaxation was evaluated by the +/-dp/dt ratio. In the control group there was impairment of myocardial relation as indicated by an increase of this index after reperfusion (from 1.05 ñ 0.05 to 1.46 ñ 0.23; P<0.05), whereas in the aortic (from 1.10 ñ 0.13 to 1.15 ñ 0.20; P>0.05) and the coronary sinus (from 1.03 ñ 0.14 to 1.08 ñ 0.16; P>0.05) groups there was no variation. Ultrastructural changes in the myocardium were negligible in all three groups at the end of reperfusion. We conclude that intermittent perfusion of a hypothermic cardioplegic solution through the coronary sinus is effective for the protection of the myocardium during total ischemia
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Index:
LILACS (Americas)
Main subject:
Cardioplegic Solutions
/
Myocardial Reperfusion
/
Myocardial Contraction
/
Myocardium
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1991
Type:
Article
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