A simultaneous ex vivo model of embryogenesis: II. Vasculogenesis.
Indian J Physiol Pharmacol
;
1999 Jul; 43(3): 315-22
Article
in English
| IMSEAR
| ID: sea-106393
ABSTRACT
Vasculogenesis was simultaneously studied with embryogenesis in in ovo chick embryo culture, which was harvested at 40 hours. Endodermal cells and vascular endothelial cells were studied using a new combination of stains, immunohistochemistry (for nuclei and basement membrane) and NADPH-diaphorase activity in whole-mounts, paraffin sections and etched semithin sections. The model can be used for the study of developmental process of blood vessels as well as embryonic physiology of blood vessels vis-a-vis organogenesis in response to different angiogenic agents, drug trials, cancer therapy by angiostatic chemicals/radiations and toxins. Considering that vasculogenesis/angiogenesis as one of the fundamental phenomena in physiology, pathophysiology, toxicology and pharmacology of developmental sciences, the model in developing embryo is presented.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Immunohistochemistry
/
Neovascularization, Physiologic
/
Culture Techniques
/
Endoderm
/
Animals
/
Models, Biological
/
NADPH Dehydrogenase
Language:
English
Journal:
Indian J Physiol Pharmacol
Year:
1999
Type:
Article
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