Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy.
J Biosci
;
2002 Jun; 27(3): 261-72
Article
in English
| IMSEAR
| ID: sea-110615
ABSTRACT
A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pituitary Gland
/
Protein Conformation
/
Carps
/
Growth Hormone
/
Molecular Sequence Data
/
Base Sequence
/
Models, Molecular
/
Amino Acid Sequence
/
Cloning, Molecular
/
Sequence Analysis, DNA
Country/Region as subject:
Asia
Language:
English
Journal:
J Biosci
Year:
2002
Type:
Article
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