Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity.
J Biosci
;
2003 Jun; 28(4): 379-82
Article
in English
| IMSEAR
| ID: sea-110683
ABSTRACT
Firefly luciferase bioluminescence (FLB) is a highly sensitive and specific method for the analysis of adenosine-5-triphosphate (ATP) in biological samples. Earlier attempts to modify the FLB test for enhanced sensitivity have been typically based on in vitro cell systems. This study reports an optimized FLB procedure for the analysis of ATP in small tissue samples. The results showed that the sensitivity of the FLB test can be enhanced several fold by using ultraturax homogenizer, perchloric acid extraction, neutralization of acid extract and its optimal dilution, before performing the assay reaction.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Time Factors
/
Coleoptera
/
Biochemistry
/
Brain
/
Female
/
Adenosine Diphosphate
/
Adenosine Triphosphate
/
Sensitivity and Specificity
/
Firefly Luciferin
/
Luciferases
Type of study:
Diagnostic study
Language:
English
Journal:
J Biosci
Year:
2003
Type:
Article
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