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DNA synthesis in the imaginal wing discs of the American bollworm Helicoverpa armigera (Hübner).
J Biosci ; 2002 Mar; 27(2): 113-20
Article in English | IMSEAR | ID: sea-110706
ABSTRACT
The effect of two insect growth regulators of plant origin viz. plumbagin and azadirachtin and the ecdysteroids 20-hydroxyecdysone, makisterone A and a phytoecdysteroid on DNA synthesis in imaginal wing discs of day 4 final instar Helicoverpa armigera larvae was studied. DNA synthesis increased with increase in time of incubation up to 8 h and decreased later without the addition of moulting hormone. Addition of 20-hydroxyecdysone supported long term acquisition of competence for DNA synthesis in the wing discs. Both DNA synthesis and protein content were drastically reduced in plumbagin and azadirachtin-treated insects. Under in vitro conditions, plumbagin had a more pronounced inhibitory effect than azadirachtin. All the ecdysteroids tested, viz. makisterone A, 20-hydroxyecdysone and the ecdysteroidal fraction from the silver fern Cheilanthes farinosa enhanced DNA synthesis.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Triterpenes / DNA / Naphthoquinones / Insect Proteins / Ecdysteroids / Limonins / Ecdysone / Ecdysterone / Insecticides / Larva Language: English Journal: J Biosci Year: 2002 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Triterpenes / DNA / Naphthoquinones / Insect Proteins / Ecdysteroids / Limonins / Ecdysone / Ecdysterone / Insecticides / Larva Language: English Journal: J Biosci Year: 2002 Type: Article