Role of reactive oxygen species in extracellular signal-regulated protein kinase phosphorylation and 6-hydroxydopamine cytotoxicity.
J Biosci
;
2003 Feb; 28(1): 83-9
Article
in English
| IMSEAR
| ID: sea-110814
ABSTRACT
A number of reports indicate the potential for redox signalling via extracellular signal-regulated protein kinases (ERK) during neuronal injury. We have previously found that sustained ERK activation contributes to toxicity elicited by 6-hydroxydopamine (6-OHDA) in the B65 neuronal cell line. To determine whether reactive oxygen species (ROS) play a role in mediating ERK activation and 6-OHDA toxicity, we examined the effects of catalase, superoxide dismutase (SOD1), and metalloporphyrin antioxidants ('SOD mimetics') on 6-OHDA-treated cells. We found that catalase and metalloporphyrin antioxidants not only conferred protection against 6-OHDA but also inhibited development of sustained ERK phosphorylation in both differentiated and undifferentiated B65 cells. However, exogenously added SOD1 and heat-inactivated catalase had no effect on either toxicity or sustained ERK phosphorylation. This correlation between antioxidant protection and inhibition of 6-OHDA-induced sustained ERK phosphorylation suggests that redox regulation of ERK signalling cascades may contribute to neuronal toxicity.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phosphorylation
/
Rats
/
Superoxide Dismutase
/
Catalase
/
Cell Differentiation
/
Oxidopamine
/
Reactive Oxygen Species
/
Mitogen-Activated Protein Kinases
/
Cell Line, Tumor
/
Enzyme Activation
Language:
English
Journal:
J Biosci
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS