Coat protein-mediated resistance against an Indian isolate of the Cucumber mosaic virus subgroup IB in Nicotiana benthamiana.
J Biosci
;
2008 Jun; 33(2): 249-57
Article
in English
| IMSEAR
| ID: sea-110828
ABSTRACT
Coat protein (CP) -mediated resistance against an Indian isolate of the Cucumber mosaic virus (CMV) subgroup IB was demonstrated in transgenic lines of Nicotiana benthamiana through Agrobacterium tumefaciens-mediated transformation. Out of the fourteen independently transformed lines developed, two lines were tested for resistance against CMV by challenge inoculations. The transgenic lines exhibiting complete resistance remained symptomless throughout life and showed reduced or no virus accumulation in their systemic leaves after virus challenge. These lines also showed virus resistance against two closely related strains of CMV. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IB member isolated from India.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Nicotiana
/
Transformation, Genetic
/
Enzyme-Linked Immunosorbent Assay
/
Immunoblotting
/
Plants, Genetically Modified
/
Agrobacterium tumefaciens
/
Cucumovirus
/
Capsid Proteins
Language:
English
Journal:
J Biosci
Year:
2008
Type:
Article
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