Reconfirmation of antimicrobial activity in the coelomic fluid of the earthworm Eisenia fetida andrei by colorimetric assay.
J Biosci
;
2003 Dec; 28(6): 723-31
Article
in English
| IMSEAR
| ID: sea-110879
ABSTRACT
A novel tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) was used in the assessment of antimicrobial activity in earthworm in the presence of phenazine methosulphate (PMS) as an electron coupling reagent. This activity was purified from the coelomic fluid of the earthworm (ECF), Eisenia fetida andrei (Oligochaeta, Lumbricidae, annelids) using a series of column chromatography techniques and was tested against three Gram-negative strains of Escherichia coli, Pseudomonas aeruginosa, Aeromonas hydrophila and three Gram-positive strains of Staphylococcus aureus, Bacillus megaterium, Arthrobacter sp., respectively. Only the pigment-free eluate of coelomic fluid of the earthworm (ECFPE) showed activity against B. megaterium amongst three isolated active fractions. The anion (DEAE-52) exchange effluent of the ECFPE was reported to have the strongest activity against P. aeruginosa amongst the three active fractions. The 20% acetonitrile eluate (AE) by Sep-Pak C18 cartridge was also tested and showed fair resistance against E. coli, P. aeruginosa and Arthrobacter sp., respectively.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oligochaeta
/
Microbial Sensitivity Tests
/
Chromatography, Gel
/
Chromatography, Ion Exchange
/
Colorimetry
/
Gram-Negative Bacteria
/
Animals
/
Anti-Bacterial Agents
Language:
English
Journal:
J Biosci
Year:
2003
Type:
Article
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