Cullin4B/E3-ubiquitin ligase negatively regulates beta-catenin.
J Biosci
;
2007 Sep; 32(6): 1133-8
Article
in English
| IMSEAR
| ID: sea-110932
ABSTRACT
Beta-catenin is the key transducer of Wingless-type MMTV integration site family member (Wnt) signalling, upregulation of which is the cause of cancer of the colon and other tissues. In the absence of Wnt signals, beta-catenin is targeted to ubiquitin-proteasome-mediated degradation. Here we present the functional characterization of E3-ubiquitin ligase encoded by cul4B. RNAi-mediated knock-down of Cul4B in a mouse cell line C3H T10 (1/2) results in an increase in beta-catenin levels. Loss-of-function mutation in Drosophila cul4 also shows increased beta-catenin/Armadillo levels in developing embryos and displays a characteristic naked-cuticle phenotype. Immunoprecipitation experiments suggest that Cul4B and beta-catenin are part of a signal complex in Drosophila, mouse and human. These preliminary results suggest a conserved role for Cul4B in the regulation of beta-catenin levels.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Transcription Factors
/
Humans
/
Animals, Genetically Modified
/
Down-Regulation
/
Drosophila Proteins
/
Cell Line, Tumor
/
Ubiquitin-Protein Ligases
/
Cullin Proteins
/
Drosophila melanogaster
/
Armadillo Domain Proteins
Language:
English
Journal:
J Biosci
Year:
2007
Type:
Article
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