The analysis of proteome changes in sunflower seeds induced by N+ implantation.
J Biosci
;
2006 Jun; 31(2): 247-53
Article
in English
| IMSEAR
| ID: sea-111021
ABSTRACT
In this work, the proteomic changes induced by N+ ion implantation were investigated using a sunflower seed model by a two-dimensional electrophoretic analysis. To further understand the changes of total protein irradiated with N+ ion, a proteomic analysis of N+ ion implantation seeds was developed. Among approximately 369 total protein spots displayed in 2-D gels, eight specific proteins were found in non-implanted seeds while four proteins were found in implanted seeds. Six proteins were used for MALDI-TOF MS analysis, of which only two had been reported before. The proteins designated as No.29 showed 23.4% homology to MADS-box transcriptional factor HAM59, while No. 279 protein had 23.20% identity to homeobox-leucine zipper protein HAHB-4. The analysis of proteome changes induced by N+ implantation could provide a new clue to studying mutation mechanism of ion implantation. To our knowledge, this is the first report about the analysis of proteome changes induced by N+ implantation in sunflower seeds.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Proteins
/
Seeds
/
Molecular Sequence Data
/
Electrophoresis, Gel, Two-Dimensional
/
Amino Acid Sequence
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteome
/
Helianthus
/
Nitrogen
Type of study:
Prognostic study
Language:
English
Journal:
J Biosci
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS