Hypervariable spacer regions are good sites for developing specific PCR-RFLP markers and PCR primers for screening actinorhizal symbionts.
J Biosci
;
2003 Jun; 28(4): 437-42
Article
in English
| IMSEAR
| ID: sea-111102
ABSTRACT
While the ribosomal RNA like highly conserved genes are good molecular chronometers for establishing phylogenetic relationships, they can also be useful in securing the amplification of adjoining hyper-variable regions. These regions can then be used for developing specific PCR primers or PCR-RFL profiles to be used as molecular markers. We report here the use of ITS region of rrn operon of Frankia for developing PCR-RFL profiles capable of discriminating between closely related frankiae. We have also made use of the ITS1 region of the nuclear rrn operon of Alnus nepalensis (D Don) for designing a PCR primer for specific amplification of nuclear DNA of this tree.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Operon
/
Phylogeny
/
Symbiosis
/
Polymorphism, Restriction Fragment Length
/
DNA
/
RNA, Ribosomal, 16S
/
RNA, Ribosomal, 23S
/
DNA Restriction Enzymes
/
Polymerase Chain Reaction
/
DNA Primers
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
English
Journal:
J Biosci
Year:
2003
Type:
Article
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