An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine.
J Biosci
;
2000 Mar; 25(1): 47-55
Article
in English
| IMSEAR
| ID: sea-111150
ABSTRACT
Japanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92 2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Immunoenzyme Techniques
/
Sensitivity and Specificity
/
Chemokines, CC
/
Encephalitis Virus, Japanese
/
Chemokine CCL22
/
Animals
/
Macrophages
/
Mice
Type of study:
Diagnostic study
Language:
English
Journal:
J Biosci
Year:
2000
Type:
Article
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