Tyrosine phosphorylation of the human guanylyl cyclase C receptor.
J Biosci
;
2000 Dec; 25(4): 339-46
Article
in English
| IMSEAR
| ID: sea-111272
ABSTRACT
Tyrosine phosphorylation events are key components of several cellular signal transduction pathways. This study describes a novel method for identification of substrates for tyrosine kinases. Co-expression of the tyrosine kinase EphB1 with the intracellular domain of guanylyl cyclase C (GCC) in Escherichia coli cells resulted in tyrosine phosphorylation of GCC, indicating that GCC is a potential substrate for tyrosine kinases. Indeed, GCC expressed in mammalian cells is tyrosine phosphorylated, suggesting that tyrosine phosphorylation may play a role in regulation of GCC signalling. This is the first demonstration of tyrosine phosphorylation of any member of the family of membrane-associated guanylyl cyclases.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phosphorylation
/
Plasmids
/
Tyrosine
/
Peptide Mapping
/
Recombinant Fusion Proteins
/
Humans
/
Immunoglobulin G
/
Precipitin Tests
/
Signal Transduction
/
Cell Line
Language:
English
Journal:
J Biosci
Year:
2000
Type:
Article
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