Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana.
J Biosci
;
2008 Mar; 33(1): 91-101
Article
in English
| IMSEAR
| ID: sea-111306
ABSTRACT
A full-length cDNA encoding ribosome-inactivating/antiviral protein (RIP/AVP)from the leaves of Bougainvillea x buttiana was isolated.The cDNA consisted of 1364 nucleotides with an open reading frame (ORF)of 960 nucleotides encoding a 35.49 kDa protein of 319 amino acids.The deduced amino acid sequence has a putative active domain conserved in RIPs/AVPs and shows a varying phylogenetic relationship to the RIPs from other plant species.The deduced protein has been designated BBAP1 (Bougainvillea x buttiana antiviral protein1).The ORF was cloned into an expression vector and expressed in E.coli as a fusion protein of approximately 78 kDa.The cleaved and purified recombinant BBAP1 exhibited ribosome-inhibiting rRNA N-glycosidase activity,and imparted a high level of resistance against the tobacco mosaic virus (TMV).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Antiviral Agents
/
Phylogeny
/
Plant Proteins
/
Tobacco Mosaic Virus
/
Recombinant Fusion Proteins
/
Protein Sorting Signals
/
Molecular Sequence Data
/
Protein Synthesis Inhibitors
/
Base Sequence
/
Gene Expression
Language:
English
Journal:
J Biosci
Year:
2008
Type:
Article
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