Overexpression of GbERF confers alteration of ethylene-responsive gene expression and enhanced resistance to Pseudomonas syringae in transgenic tobacco.
J Biosci
;
2006 Jun; 31(2): 255-63
Article
in English
| IMSEAR
| ID: sea-111322
ABSTRACT
GbERF belongs to the ERF (ethylene responsive factor) family of transcription factors and regulates the GCC-box containing pathogen-related (PR) genes in the ethylene signal transduction pathway. To study the function of GbERF in the process of biotic stress, transgenic tobacco plants expressing GbERF were generated. Overexpression of GbERF did not change transgenic plant's phenotype and endogenous ethylene level. However, the expression profile of some ethylene-inducible GCC-box and non-GCC-box containing genes was altered, such as PR1b, PR2, PR3, PR4, Osmotin, CHN50, ACC oxidase and ACC synthase genes. These data indicate that the cotton GbERF could act as a transcriptional activator or repressor to regulate the differential expression of ethylene-inducible genes via GCC and non-GCC cis-elements. Moreover, the constitutive expression of GbERF in transgenic tobacco enhanced the plant's resistance to Pseudomonas syringae pv tabaci infection. In conclusion, GbERF mediates the expression of a wide array of PR and ethylene-responsive genes and plays an important role in the plant's response to biotic stress.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phenotype
/
Plant Proteins
/
Seeds
/
Nicotiana
/
Transcription Factors
/
Transformation, Genetic
/
Nuclear Proteins
/
Base Sequence
/
Signal Transduction
/
Plants, Genetically Modified
Language:
English
Journal:
J Biosci
Year:
2006
Type:
Article
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